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Physiological functions of GPx2 and its role in inflammation-triggered carcinogenesis

Moreover, tumor-derived exosomes are able to promote cancer cell migration and metastasis, containing various kinds of integrin, such as exosomal integrins v 6 for prostate cancer (Carmine et al., 2015), 6 4 and 6 1 for lung cancer, and v 5 for liver cancer metastasis (Hoshino et al., 2015)

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After washing with PBS, samples were incubated with Alexa Fluor 488- or 555-conjugated secondary antibodies (Invitrogen, California, A-21202/A-31572, USA) for 1 h at room temperature in the dark and stained with 4,6-diamidino-2-phenylindole (DAPI)